B码设备中的GPS时间信号接口     

张季珍 《时间频率学报》1999,22(1):18-21

ＰＯ９７型ＧＰＳ／Ｂ码解调器把ＧＰＳ技术融入Ｂ码设备中,用于自动定时定位和精密时间同步。该设备的ＧＰＳ时码接口解出ＧＰＳ时间信号,并同步所有的时标信号和时间码信号。主要介绍了该接口的硬件和软件的实现过程。  相似文献   

标准地址共享服务平台的设计与实现     

郭丽  祝士杰 《测绘与空间地理信息》2014,(10):175-177

在对标准地址库系统进行设计的基础上,详细阐述了标准地址共享服务平台——＂一库三平台＂的系统设计思路。建立了杭州市标准地址共享服务平台,即＂一库三平台＂,具体为：一个数据库——标准地址库,三个服务平台——标准地址云服务平台、标准地址监控管理平台、标准地址应用服务平台;该系统为杭州市各级政府各业务部门地理信息应用提供了统一的标准基础信息数据和标准地址库服务;同时,利用地址匹配与业务关联采集技术,实现业务信息资源与标准地址库的有机关联,实现了业务信息数据海量存储与应用,为政府及社会公众在工作和生活中提供翔实的检索、定位信息和云平台服务。  相似文献   

Derivation of an urban materials spectral library through emittance and reflectance spectroscopy     

《ISPRS Journal of Photogrammetry and Remote Sensing》2014

Recent advances in thermal infrared remote sensing include the increased availability of airborne hyperspectral imagers (such as the Hyperspectral Thermal Emission Spectrometer, HyTES, or the Telops HyperCam and the Specim aisaOWL), and it is planned that an increased number spectral bands in the long-wave infrared (LWIR) region will soon be measured from space at reasonably high spatial resolution (by imagers such as HyspIRI). Detailed LWIR emissivity spectra are required to best interpret the observations from such systems. This includes the highly heterogeneous urban environment, whose construction materials are not yet particularly well represented in spectral libraries. Here, we present a new online spectral library of urban construction materials including LWIR emissivity spectra of 74 samples of impervious surfaces derived using measurements made by a portable Fourier Transform InfraRed (FTIR) spectrometer. FTIR emissivity measurements need to be carefully made, else they are prone to a series of errors relating to instrumental setup and radiometric calibration, which here relies on external blackbody sources. The performance of the laboratory-based emissivity measurement approach applied here, that in future can also be deployed in the field (e.g. to examine urban materials in situ), is evaluated herein. Our spectral library also contains matching short-wave (VIS–SWIR) reflectance spectra observed for each urban sample. This allows us to examine which characteristic (LWIR and) spectral signatures may in future best allow for the identification and discrimination of the various urban construction materials, that often overlap with respect to their chemical/mineralogical constituents. Hyperspectral or even strongly multi-spectral LWIR information appears especially useful, given that many urban materials are composed of minerals exhibiting notable reststrahlen/absorption effects in this spectral region. The final spectra and interpretations are included in the London Urban Micromet data Archive (LUMA; http://LondonClimate.info/LUMA/SLUM.html).  相似文献   

对虾白斑综合症病毒极早期基因启动子筛选文库的构建     

林梵宇  徐丽美  杨丰 《台湾海峡》2010,29(2):184-188

对虾白斑综合症病毒（white spot syndrome virus,WSSV）是危害对虾养殖业的主要病原,而WSSV极早期基因对病毒功能基因的转录翻译和调控起着至关重要的作用.本研究将WSSV基因组以超声法随机断裂至400～900bp DNA片段,并插入启动子缺失且多克隆位点下游具有报告基因的载体来构建文库,转染昆虫细胞Hi5.倒置荧光显微镜观察发现部分转染后的细胞有增强型绿色荧光蛋白（EGFP）的表达,表明这部分细胞中的DNA片段可能具有极早期启动子活性.流式细胞仪分析显示,这部分荧光细胞占总细胞的比例为0.35%.由于转染效率为50%,可认为占比0.7%的WSSV基因组随机片段能在Hi5细胞中激发下游报告基因的转录翻译,可能具有极早期基因启动子活性.这对于进一步筛选和鉴定WSSV极早期基因及开展极早期启动子研究具有重要的意义.  相似文献   

二维码定位技术支持下的室内导航电子地图的设计与实现——以武汉大学图书馆为例     

庄莹  黄丽娜  郑恒杰  刘统硕  康雨豪 《测绘通报》2017,(5):125

随着大型复杂公共室内空间逐渐增多,室内导航已成为当前基于位置服务（LBS）领域的研究热点。本研究结合空间定位技术和移动地图技术,设计和开发了一种面向复杂室内空间的导航电子地图,利用二维码技术进行准确的室内空间定位,并以武汉大学图书馆为样区,设计了一套面向复杂室内空间结构的位置编码规则。在此基础上,开发和实现了可提供图书馆室内定位与最优路径推送的手机端导航电子地图。  相似文献   

基于ArcGIS建立地震救援现场地图符号库     

魏昌盛  陈维锋  黄丁发  郭红梅  雷秋霞 《地理空间信息》2012,10(4):18-19,22

针对地震救援这一特殊事件,按照一定的原则完成地震救援现场符号的分类,并对涉及的事物事件根据相应的原则完成符号设计。运用ArcGIS软件的2种符号库建立方法生成应用于地震救援现场的专题符号库,并对2种方法进行了简单比较。  相似文献   

Construction of a Metagenomic DNA Library of Sponge Symbionts and Screening of Antibacterial Metabolites   总被引：1，自引：0，他引：1  

CHEN Juan ZHU Tianjiao LI Dehai CUI Chengbin FANG Yuchun LIU Hongbing LIU Peipei GU Qianqun ZHU Weiming 《中国海洋大学学报(英文版)》2006,5(2):119-122

To study the bioactive metabolites produced by sponge-derived uncultured symbionts, a metagenomic DNA library of the symbionts of sponge Gelliodes gracilis was constructed. The average size of DNA inserts in the library was 20 kb. This library was screened for antibiotic activity using paper disc assaying. Two clones displayed the antibacterial activity against Micrococcus tetragenus. The metabolites of these two clones were analyzed through HPLC. The result showed that their metabolites were quite different from those of the host E. coli DH5α and the host containing vector pHZ132. This study may present a new approach to exploring bioactive metabolites of sponge symbionts.  相似文献   

南钢无锡金鑫轧钢厂设备基座搬迁精密工程测量方法     

姚成武 《现代测绘》2009,32(4):31-33

本文主要介绍了针对无锡轧钢厂设备基座的精密工程测量,并结合测量中的实际情况,重点提出如何避免和降低误差对大型机械设备测量的影响.  相似文献   

Microbial diversity in polluted harbor sediments II: Sulfate-reducing bacterial community assessment using terminal restriction fragment length polymorphism and clone library of dsrAB gene     

Wen Zhang  Lin-sheng Song  Jang-Seu Ki  Chun-Kwan Lau  Xiang-Dong Li  Pei-Yuan Qian 《Estuarine, Coastal and Shelf Science》2008

Sulfate-reducing bacteria (SRB) are important regulators of a variety of processes in coastal marine sediments regarding organic matter turnover, biodegradation of pollutants, and sulfur and carbon cycles. Yet their community compositions have not been investigated in polluted harbor sediments. This study described the diversity and spatial variation of SRB communities in surface sediments in Victoria Harbor, Hong Kong. The spatial variation of SRB communities was described by terminal restriction fragment length polymorphism (T-RFLP). The results showed that the most diversified terminal restriction fragments were found at polluted sites. In addition, cluster analysis indicated that although the SRB communities were different at the two polluted sites, they were still more similar to each other than to the two more distant reference sites. Based on a dsrAB clone library constructed at a polluted site, diversified SRB were found, represented by 30 Operational Taxonomic Units (OTUs). Upon comparisons among the SRB sequences detected from this study and those in the GenBank, five clades of SRB were found. Three clades belonged to the known families Desulfobacteraceae, Desulfobulbaceae, and Syntrophobacteriaceae. The majority of sequenced clones, which distantly related to sequences in the GenBank, constituted the remaining two unclassified groups, suggesting unique SRB members related to the polluted harbor environment. Statistical analyses indicated that estimated SRB richness correlated with environment factors such as sulfur content, acid volatile sulfate, and redox potential.  相似文献   

南极欺骗岛土壤细菌群落结构和多样性分析          下载免费PDF全文

王健孙云峰  李晨曦吴玥李艳红  杨志伟 《极地研究》2017,29(4):486-495

采用基于16S r DNA克隆文库的非培养方法和传统培养方法相结合的手段,对南极欺骗岛土壤细菌群落结构及多样性进行了初步分析。对来自16S r DNA克隆文库的118个阳性克隆进行测序和序列比对,结果显示细菌来自放线菌门(Actinobacteria)、变形杆菌门(Proteobacteria)和拟杆菌门(Bacteroidetes)3大门的39个操作分离单元(OTU),其中优势菌群为放线菌门和变形杆菌门,分别占65.25%和28.81%。放线菌门的主要优势属为鱼孢菌属(Sporichthya)、类诺卡式氏菌属(Nocardioides)、束缚菌属(Conexibacter)、Gaiella和节杆菌属(Arthrobacter)。变形杆菌门的主要优势属为鞘氨醇单胞菌属(Sphingomonas)、硫杆菌属(Thiobacillus)、寡养单胞菌属(Stenotrophomonas)和热单胞菌属(Thermomonas)。采用6种培养基对细菌进行培养和分离,共得到57株菌,来自放线菌门、变形杆菌门、拟杆菌门和厚壁菌门4大门的13个OTU,其中优势菌群为放线菌门和变形杆菌门,分别占57.89%和31.58%。优势属是放线菌门的节杆菌属(Arthrobacter)、雷夫松氏菌属(Leifsonia)和变形杆菌门的假单胞菌属(Pseudomonas)。本论文为研究欺骗岛土壤细菌多样性以及有益菌种资源的开发和利用奠定了基础。  相似文献